Services

OsperyBio uses the Bird of Prey DNA Construction and Delivery System (BoP). It standardizes complex DNA construct development while being modality, delivery, complexity and size agnostic with a simple set of Design Rules. All manufacturing and Design is done exclusively in the United States. Login to the dashboard to start a service request.

Pricing

  • A single module step for construction starts at $169 with each additional step adding another starting amount of $169.
  • If we already have the construct made, then it is just the base value of $169.
  • BoPizing is priced at $150.
  • Custom services require more communication and every order or project is quoted for review.

Workflows

Therapy Development

Use BoP to add, delete, and/or modify genes.

Step One

Define your desired therapy modalities:

  • Recombinant Protein
  • Therapeutic RNA
  • Non-viral Gene Therapy
  • Viral Gene Therapy
  • Organell (ex: exosome, mitochondria)
  • GM-Cell

Step Two

Chose any number of genes from our library.

OR

Submit your proprietary, non-wildtype "Gene(s) of Interest" for "BoP-ization" and subsequent modular assembly.

Step Three

Submit any design configuration of genes for assembly.

Step Four

Choose your desired Gene Delivery Vector Harnesses.

Disease Model Vectors

Construct a vector to model a disease state.

Step One

Select Mechanism to induce disease state.

  • Change genomically encoded loci:
    • Knock-out (KO)
    • Knock-in of Mutated Alleles (KI)
  • Introduction of exogenous modifying components:
    • Knock-down (KD) of Target Genes(s) via bioRNAs
    • OverExpression of Key Effector(s)

Step Two

Select model system:

  • Cell
  • Organoid
  • Organism

Step Three

Design Assays and complementary reagents.

Standardized Reporter System Vectors

Build a system to monitor gene activity.

Step One

Identify your desired reporter(s) from our library:

  • Fluorescent protein based (ex: GFP or RFP)
  • Enzymatic (ex: Luciferase, Alkaline Phosphatase)

OR

Submit your desired reporter(s) to be converted into Bird of Prey.

Step Two

Choose from various regulatory promoter elements (including both 5' and 3' elements):

  • Broadly constitutive expression
  • Broadly ubiquitous expression
  • Cell-type specific expression
  • Cell-state specific expression (ex: hypoxia-induced)

Step Three

Chose:

  • Genomic Status:
    • Transient-episomal
    • Stable
      • Integrase-mediated
      • Homologous Recombination-mediated
  • Delivery:
    • Viral
    • Non-Viral

RNA IVT

Express your desired protein of interest.

Step One

Identify your therapeutic targets and desired therapy modality:

  • Therapeutic RNA
  • Recombinant Protein

Step Two

Chose any number of genes from our library.

OR

Submit your proprietary, non-wildtype "Gene(s) of Interest" for "BoP-ization" and subsequent modular assembly.

Step Three

Introduce your BoP-ized Gene of Interest into RNA synthesis backbone for cell-free manufacturing.

*Applications: RNA-based vaccine development; transient gene therapy.

Protein Based Therapy Development Vectors

Express your desired protein of interest.

Step One

Identify your therapeutic targets and desired therapy modality:

  • Therapeutic RNA
  • Recombinant Protein

Step Two

Chose any number of genes from our library.

OR

Submit your proprietary, non-wildtype "Gene(s) of Interest" for "BoP-ization" and subsequent modular assembly.

Step Three

Introduce your BoP-ized Gene of Interest into RNA synthesis backbone for cell-free manufacturing.

*Applications: RNA-based vaccine development; transient gene therapy.

Step Four

Introduce your BoP-ized Gene of Interest into PROTEIN EXPRESSION systems-specific vector harnesses. (ex: bacteria, yeast, mammalian cells, plant cells, algae, and more.)

*Selection of optimal expression system dependent on protein complexity, size, folding structure and post-translational modifications.